The Expression of Phospholipase C-gamma1 and Its Cellular Characteristics.
- Author:
Dong Young NOH
1
;
Han Sung KANG
;
Young Chul KIM
;
In Ae PARK
;
Yeo Kyu YONG
;
Seung Keun OH
;
Kuk Jin CHOE
Author Information
1. Department of Surgery, Seoul National University College of Medicine.
- Publication Type:Original Article
- Keywords:
Breast cancer;
PLC-gamma1;
Prognostic factor;
E-cadherin
- MeSH:
Breast Neoplasms;
Cadherins;
Humans;
Phospholipases*;
Protein-Tyrosine Kinases;
Biomarkers, Tumor
- From:Journal of the Korean Cancer Association
1998;30(3):457-463
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The activation of phospholipase C(PLC) is one of the early cellular events in various growth process, including malignant transformation. PLC-gamma1 is activated through direct interaction with growth factor receptor tyrosine kinase. MATERIAL AND METHODS: Using immunoblot assay, we evaluated overexpression of PLC-gamma1 expression in twenty human breast cancer tissues. It was also determined whether there was any connection between other prognostic factors(numbers of metastatic axillary nodes, nuclear and histological grade, c-erbB2, p53 and E-cadherin) and the overexpression of PLC-gamma1 protein. RESULTS: Seventeen of 20 breast cancer tissues showed overexpression of PLC-gamma1, which was corresponded to that seen on the immunohistochemistry( kappa= 0.8275, p = 0.003). Of 3 tumor markers, immunohistochemically determined, positive expression of E-cadherin only was associated with PLC-gamma1 protein overexpression in a range of statistical significance (p=0.045, kappa=0.607). CONCLUSION: PLC-gamma1 overexpression might be pathogenic trigger involved in breast cancer and the relationship between expression of E-cadherin and PLC-gamma1 would require further elucidation.
