Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin Ⅰ-converting enzyme in vitro
- Author:
Crown Olajusi OLAMIDE
1
;
Olayeriju Sam OLANREWAJU
;
Kolawole Oluwaseyi AYODELE
;
Akinmoladun Clement AFOLABI
;
Olaleye Tolulope MARY
;
Akindahunsi Akintunde AFOLABI
Author Information
1. Department of Biochemistry
- Keywords:
Angiotensin converting enzyme;
Blood pressure;
FAPGG;
Mixed type inhibition;
Parinari curatellifolia
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(12):1079-1084
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the possible inhibitory potentials and mechanism by Mobola plum (Parinari curatellifolia) seeds crude methanol (CE) and flavonoid-rich (FE) extracts on angiotensin-1-converting enzyme (ACE I).Methods:The sensitivity and kinetic model of inhibition of CE and FE on ACE I using N-[3-(2-furyl)-acryloyl]-Phe-Gly-Gly as enzyme substrate for ACE I was evaluated by Michealis Menten approach.The inhibition mechanism was explored from LineweaverBurk model and IC50 was determined from Cheng-Prusoff empirical analysis.Results:The IC50 of CE and FE were 13.54 and 39.38 μg/mL,respectively.Both extracts exhibited mixed type inhibition with the inhibitory constant (Ki) of CE was between 0.38 and 0.37 μg/mL while that of FE showed a two-fold increase (1.62 μg/mL and 0.28 μg/mL).FE on ACE I demonstrated positive cooperativity with a Hill's coefficient of 1.89.Conclusions:The study reveals the superior ACE I inhibitory potential of CE over FE and suggest that mixed inhibition pattern of the enzyme might be the underlying mechanism of antihypertensive activity.
