Açaí Berries Inhibit Colon Tumorigenesis in Azoxymethane/Dextran Sulfate Sodium-Treated Mice.
- Author:
Yoon Jin CHOI
1
;
Yoon Jeong CHOI
;
Nayoung KIM
;
Ryoung Hee NAM
;
Seonmin LEE
;
Hye Seung LEE
;
Ha Na LEE
;
Young Joon SURH
;
Dong Ho LEE
Author Information
- Publication Type:Original Article
- Keywords: Açaí berry; Colorectal neoplasms; Anti-inflammatory; Proapoptotic
- MeSH: Adenoma; Animals; Azoxymethane; Carcinogenesis*; Colon*; Colonic Neoplasms; Colorectal Neoplasms; Cyclooxygenase 2; Cytokines; Enzyme-Linked Immunosorbent Assay; Fruit*; Humans; Immunoblotting; In Vitro Techniques; Incidence; Interleukin-6; Interleukins; Lymphoma, B-Cell; Macrophages; Mice*; Necrosis; Peroxidase; Proliferating Cell Nuclear Antigen; RAW 264.7 Cells; Sodium
- From:Gut and Liver 2017;11(2):243-252
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/AIMS: The aim of this study was to investigate the protective effect of açaí against azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colorectal cancer development. METHODS: The effect of açaí on tumorigenesis was assessed by evaluating tumor incidence, multiplicity and invasiveness in the mouse colon. The levels of myeloperoxidase (MPO) and proinflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-1β, and IL-6) were measured via enzyme-linked immunosorbent assay. Protein levels of cyclooxygenase 2 (COX-2), proliferating cell nuclear antigen (PCNA), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated death promoter (Bad) and cleaved-caspase-3 were assessed by immunoblotting. RESULTS: Administration of pellets containing 5% açaí powder reduced the incidences of both colonic adenoma and cancer (adenoma, 23.1% vs 76.9%, respectively, p=0.006; cancer, 15.4% vs 76.9%, respectively, p=0.002). In the açaí-treated mice, the MPO, TNF-α, IL-1β and IL-6 levels in the colon were significantly down-regulated. Açaí inhibited PCNA and Bcl-2 expression and increased Bad and cleaved-caspase-3 expression. In vitro studies demonstrated that açaí treatment reduced lipopolysaccharide-induced expression of TNF-α, IL-1β, IL-6 and COX-2 in murine macrophage RAW 264.7 cells. CONCLUSIONS: Açaí demonstrated protective effects against AOM/DSS-induced colon carcinogenesis, which suggests that the intake of açaí may be beneficial for the prevention of human colon cancer.
