Analysis of genetic susceptibility to proliferative diabetic retinopathy in Han patients with type 2 diabetes from Southern China by whole exome sequencing and SnaPshot
10.3760/cma.j.issn.2095-0160.2018.10.008
- VernacularTitle:全外显子组测序联合SnaPshot技术分析华南地区汉族人增生性糖尿病视网膜病变的遗传易感性
- Author:
Jie XIE
1
;
Zhengxuan JIANG
;
Liang ZHANG
;
Ying CUI
;
Jun WANG
;
Aiping ZHANG
;
Qianli MENG
Author Information
1. 华南理工大学附属广东省人民医院眼科
- Keywords:
Proliferative diabetic retinopathy;
Han nationality;
Genetic susceptibility;
Whole exome sequencing;
SnaPshot
- From:
Chinese Journal of Experimental Ophthalmology
2018;36(10):774-779
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the genetic susceptibility of proliferative diabetic retinopathy (PDR) in Han patients with type 2 diabetes from Southern China.Methods A cross-sectional study was performed under the informed consent of the patients.Patients with type 2 diabetes in the Dongguan Eye Study from September 2011 to February 2012 and relative patients treated in Guangdong General Hospital from July 2017 to March 2018 were included in this study,including 100 patients with diabetes mellitus(DM) and 120 patients with PDR.Whole exome sequencing was used to identify DNA mutation in peripheral blood samples from 22 type 2 diabetic patients without retinopathy (DM group) and 23 diabetic patients with PDR (PDR group).Genotype and allele of the nine selected single-nucleotide polymorphisms (SNPs) were tested and analyzed by SnaPshot technology in another 78 DM patients without retinopathy and 97 PDR patients.Results A total of 75 SNPs were associated with PDR (P＜0.01),involving 53 genes.Eleven gene loci were in the exon region and 7 were non-synonymous mutations.Nine exon loci of 8 genes with significant differences were screened out for the verification.SnaPshot SNP genotyping technique found that there were no significant differences in allele and genotype frequency in the nine selected SNPs between PDR group and DM group (all at P＞0.05).However,7 haplotypes distribution frequencies were significantly different between PDR group and DM group (all at P＜0.01).Hapl and Hap4 might reduce the risk of PDR (both at OR＜1,P＜0.05),and Hap2 might increase the risk of PDR (OR＞ 1,P＜0.05).Conclusions The occurrence of PDR probably has a genetic susceptibility in type 2 DM patients of Han nationality in Southern China.
