Preparation of chitosan nano-particles loaded tetrandrine and its inhibitory effects on the proliferation of human pterygium fibroblast cells
10.13389/j.cnki.rao.2018.0052
- VernacularTitle:载汉防己甲素壳聚糖纳米微球的制备及其对人翼状胬肉细胞增殖的抑制作用
- Author:
Zhi HUANG
1
;
Huai-Sheng ZHOU
;
Yang-Yang XU
;
Dan HU
;
Xiao ZHENG
;
Chi ZHANG
Author Information
1. 佛山市第一人民医院眼科
- Keywords:
tetrandrine;
chitosan;
nano-particle;
pterygium
- From:
Recent Advances in Ophthalmology
2018;38(3):226-229,234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of chitosan nano-particles loaded tetrandrine (Tet) on proliferation of cultured pterygium fibroblasts.Methods The deoxycholic acid-modified chitosan (DAMC) derivative was synthesized through amidation reaction,and their properties were investigated.The viability of human pterygium fibroblasts (HPF) was evaluated by cell counting kit-8 (CCK-8) assay after cells were interacted with Tet/DAMC nano-particles on day 1,3 and 5.Results The synthesized chitosan derivative and Tet formed drug-loaded nano-particles,and the agent-loading capacity was approximately 76％,and the sizes of agent-loaded nano-particles were 50-500 nm,with Zeta potential values being positive.The result of in vitro drug release experiment indicated that the nano-particles constantly released Tet in a controlled manner within 48 h.The viability of HPF in Tet group was (60.70 ± 2.30) ％,(50.22 ± 2.35) ％,(21.99 ± 2.07) ％ on day 1,3,5,respectively,but the corresponding data in Tet/DAMC group was (79.77 ±2.09)％,(63.24 ±2.83)％,(40.28 ± 1.19)％,respectively.The CCK-8 assay demonstrated that the Tet/DAMC nano-particles could inhibit HPF proliferation,and presented lower toxicity than Tet alone.Conclusion Chitosan nano-particles loaded tetrandrine exhibits a sustained agent-release behavior,which has obvious inhibitory effects on the proliferation of human pterygium fibroblasts,but its cytotoxicity is significantly lower than the original drug's,thereby possessing a great promise for improving the outcome of Tet for the prevention of pterygium recurrence.
