Effects of bradykinin on the proliferation of rabbit corneal endothelial cells and the expression of tight junction-related proteins ZO-1 and ZONAB
10.13389/j.cnki.rao.2018.0025
- VernacularTitle:缓激肽对体外培养兔角膜内皮细胞增殖状况及对闭锁小带蛋白-1与相关性核酸结合蛋白表达的影响
- Author:
Li-Xian HE
1
;
Yi SHAO
;
Xue-Xiang ZOU
;
Shuang-Shuang ZHOU
;
Ying ZHOU
;
Er-Hua LIU
;
Gang TAN
Author Information
1. 南华大学附属第一医院眼科
- Keywords:
bradykinin;
rabbit corneal endothelial cells;
proliferation;
ZO-1;
ZONAB
- From:
Recent Advances in Ophthalmology
2018;38(2):116-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of bradykinin (BK) on the proliferation of rabbit corneal endothehal ceils (RCECs) and the expression of tight junction-related proteins zonula occludens-1 (ZO-1) and zonula occludens-1-associated nucleic-acid-binding protein (ZONAB),and to explore the underlying mechanisms of BK on cell proliferation in corneal endothelium.Methods RCECs at logarithmic growth phase were treated with different concentrations of BK (0.01,0.1,1,10 μmol · L-1) BK group,with the controls left untreated.Morphological changes of cells in each group were examined under phase-contrast microscope,and MTT assays were used to detect cell proliferation at 24 h,48 h,72 h,96 h after BK treatment.And,at 72 h,the expression levels of ZO-1 and ZONAB protein were determined by Western blot.Results After 72 h of treatment,the cells in each group were fused into pieces and closely linked into a monolayer;but after 96 h,the growth of the cells was restricted,with the intercellular space become larger and the cells exfoliated.Compared with the control group,BK induced a significant increase of absorbance value and cell viability,and the differences were statistically significant (all P ＜ 0.001),and the promoting effects showed a concentration-dependent manner,and 1 μmol · L-1 BK demonstrated the strongest regulative effect (P ＜ 0.001).Western blot results showed that BK upregulated the expression of ZO-1 and ZONAB protein in a concentration-dependent manner.Conclusion BK can stimulate the proliferation of RCECs in a time-and concentration-dependent manner,and the mechanisms are probably associated with ZO-1/ZONAB-mediated signaling pathway.
