Expression of phosphatidic acid phosphatase type 2 domain containing 1A in different human colorectal cancer cells
10.7683/xxyxyxb.2018.03.001
- VernacularTitle:磷脂酸磷酸酶2域1A在不同结直肠癌细胞中的表达
- Author:
Pei-Qiong GAO
1
;
Guo-Yang HE
;
Si-Jia FENG
;
Cui-Cui ZHANG
;
Zhi-Hui WANG
;
Rui FAN
;
Yang-Lin WANG
;
Xiang-Nan ZHANG
;
Fei ZHAO
;
Bei-Xi WANG
;
Xin-Lai QIAN
;
Zhi-Qing YUAN
Author Information
1. 新乡医学院基础医学院病理学教研室
- Keywords:
colorectal cancer;
phosphatidic acid phosphatase type 2 domain containing 1A;
mRNA
- From:
Journal of Xinxiang Medical College
2018;35(3):163-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance of the expression of phosphatidic acid phosphatase type 2 domain containing 1A(PPAPDC1A) in human colorectal cancer cell lines.Methods The high metastatic potential cells LOVO,SW620 and low metastatic potential cells SW480,RKO,HCT116 and DLD-1 were cultured,the expression of PPAPDC1A mRNA and protein in different colorectal cancer cells in logarithmic growth period was detected by real-time quantitative polymerase chain reaction and Western blot.Results There were significant differences in the expressions of PPAPDC1A mRNA and protein among the six human colorectal cancer cells (F =41.213,344.1 16;P ＜ 0.05).The expression of PPAPDC1 A mRNA and protein in highly metastatic potential cells LOVO and SW620 was significantly higher than that in DLD-1,HCT116,RKO and SW480 cells (P ＜0.05).The expression of PPAPDC1A protein in LOVO cells with high metastatic potential was significantly higher than that in SW620 cells(P ＜ 0.05).The expression of PPAPDC1A protein in DLD-1 cells was significantly higher than that in HCT116,RKO and SW480 cells (P ＜0.05).The expression of PPAPDC1 A protein in HCT116 cells with low metastatic potential was significantly higher than that in RKO and SW480 cells (P ＜ 0.05).The expression of PPAPDC1 A protein in RKO cells was significantly higher than that in SW480 cells (P ＜ 0.05).There was no significant difference in the expression of PPAPDC1A mRNA between LOVO and SW620 cells (P ＜ 0.05).There was no significant difference in the expression of PPAPDC1A mRNA between SW480,RKO,HCT116 and DLD-1 cells (P＜ 0.05).Conclusion PPAPDC1A expresses differentially in colorectal cancer cell lines,which may be involved in the invasion and metastasis of colorectal cancer.
