Application of erlotinib based targeting fluorescent probe in the detection of lung cancer
10.7683/xxyxyxb.2018.01.005
- VernacularTitle:以埃罗替尼为靶向基团的荧光探针在肺癌检测中的应用
- Author:
Shu-Min ZHOU
1
;
Yan-Fang SONG
;
Xin-Lai QIAN
;
Jia-Bin WEI
Author Information
1. 周口市中心医院病理科
- Keywords:
erlotinib;
lung cancer;
fluorescent probe;
cellular imaging
- From:
Journal of Xinxiang Medical College
2018;35(1):22-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application of erlotinib based targeting fluorescent probe in the detection of lung cancer.Methods The erlotinib based targeting fluorescent probe was prepared.The lung cancer cells of A-549 were selected as experimental group,and cervical cancer cells of CaSki,SiHa and C33-A were selected as control group.The A-549,CaSki,SiHa and C33-A cells were identified by 0.1 × 10-6,1 × 10-6,10 × 10-6 mol · L-1 fluorescent probe;the identification ability of erlotinib based targeting fluorescent probe on cells in the two groups was observed under the inverted fluorescence microscope.Results When the concentration of fluorescent probe was 10 × 10-6 mol · L-1,the strong fluorescence signal was observed in A-549 and CaSki cells,but the fluorescence signal was not observed almost in SiHa and C33-A cells.When the concentration of fluorescent probe was 1 × 10-6 mol · L-1,the strong fluorescence signal was observed in A-549 cells;the weak fluorescence signal was observed in C33A cells;the fluorescence signal was not observed almost in SiHa and C33-A cells.When the concentration of fluorescent probe was 0.1 × 10-6 mol · L-1,the strong fluorescence signal was observed in A-549 cells;the fluorescence signal was not observed almost in CaSki,SiHa and C33-A cells.Conclusion Erlotinib based targeting fluorescent probe can specifically recognize lung cancer A-549 cells.
