A study on the correlation of miRNA in breast milk and neonatal breast milk associated jaundice
10.3760/cma.j.issn.2096-2932.2018.03.003
- VernacularTitle:母乳微小RNA与新生儿母乳性黄疸的相关性研究
- Author:
Rui HU
1
;
Jianhua SUN
;
Liqing XU
;
Jian WANG
;
Wenqiong NI
;
Yaxuan LI
;
Jing LI
Author Information
1. 200127,上海交通大学医学院附属上海儿童医学中心新生儿科
- Keywords:
Milk,human;
RNA;
Jaundice,neonatal;
High-throughput nucleotide sequencing;
Immunoregulation
- From:Chinese Journal of Neonatology
2018;33(3):170-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship of microRNA (miR) in breast milk and neonatal breast milk associated jaundice.Method From Sep.to Dec.2016,neonates with severe hyperbilirubinemia caused by breast milk were selected as the observation group,and breast-fed neonates without jaundice were selected as the control group.Their breast milk were collected,and the expression profile of miR in the breast milk was examined using miR sequencing.The variation of miR profile was screened using bioinformatics method,and miR related to neonatal breast milk associated jaundice was studied and the target genes were predicted.Result The breast milk contained many miRs associated with immunity regulation and metabolism,including miR-148-3p,miR-30a-5p,miR-146-5p,let-7f-5p,miR-181-5p,miR-22-3p,and miR-182-5p.The expressions of miR-30a-5p,miR-146a-5p and miR-141-3p in the observation group were significantly higher than the control group,and the differences were 2.600,2.038 and 1.899-fold,respectively.Uridine diphosphate glucuronosyl transferase1A1 gene was one of the target genes of miR-141-3p.Conclusion Breast milk miR may influence the growth and development,immunity regulation and metabolism of newborns.Some miRs,such as miR-141-3p,may be correlated with neonatal breast milk associated jaundice.
