The effect of Saccharomycesboulardii on interleukin-17,interleukin-10 and transforming growth factor-β1 of inflammatory bowel disease rat
10.3760/cma.j.issn.1673-4912.2018.10.010
- VernacularTitle:布拉氏酵母菌对炎症性肠病小鼠白细胞介素-17,白细胞介素-10,转化生长因子-β1表达的影响
- Author:
Guina WANG
1
;
Zhiqin MAO
Author Information
1. 中国医科大学附属盛京医院小儿消化内科
- Keywords:
Saccharomyces boulardii;
Inflammatory bowel disease;
Rats;
Interleukin-17;
Interleu-kin-10;
Transforming growth factor-β1
- From:
Chinese Pediatric Emergency Medicine
2018;25(10):757-761
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Saccharomyces boulardii(SB) on interleukin-17, interleukin-10 and transforming growth factor-β1 of inflammatory bowel disease ( IBD ) rats which were induced by trinitro-benzene-sulfonic acid( TNBS) . Methods Balb/c female rats were randomly divided into three groups,normal group,TNBS group and TNBS+SB group. TNBS method was used to set up IBD rat models. TNBS group:the mice were injected by 5％ TNBS 0. 1 ml+50％ ethanol 0. 1 ml per mouse from rectum for 5 days,and then were filled in stomach by normal saline 0. 1 ml on the 2nd day for 2 weeks;TNBS+SB group:the mice were injected by 5％ TNBS 0. 10 ml+50％ ethanol 0. 10 ml per mouse from rectum for 5 days,and then were filled in stomach by SB (SB no less than 109 CFU/ml) 0. 1 ml on the 2nd day for 2 weeks;the normal group:the mice were injected by 50％ ethanol 0. 2 ml per mouse from rectum for 5 days, and then were filled in stomach by normal saline 0. 1 ml on the 2nd day for 2 weeks. At the 15th day,the general situation of mice were observed,serum and colon tissue was collected. Alterations of colon inflamma-tion were observed by means of haematoxylin-eosin ( HE);the level of IL-17,IL-10 and TGF-β1 in serum were evaluated by enzyme linked immunosorbent assay (ELISA). The level of IL-17,IL-10 and TGF-β1 in colon tissue was evaluated by immunohistochemistry. Results The IL-17 level of serum and colon tissue in-creased(P<0. 05),but IL-10(P<0. 05) and TGF-β1(P<0. 05)decreased in TNBS group compared with those in the normal group. The level of IL-17 in TNBS+SB group was lower than that in TNBS group( P<0. 05),but the levels of IL-10(P<0. 05) and TGF-β1(P<0. 05)were higher than those in TNBS group. Compared with the normal group,the level of IL-17 in TNBS+SB group increased ( P<0. 05 ) , but IL-10 (P<0.05) and TGF-β1(P <0.05)decreased. Conclusion The expression of cytokine IL-17 increase, IL-10 and TGF-β1 decrease in peripheral blood and colon tissue of IBD. SB may ameliorate the intestinal inflammatory response of IBD by balancing the expression of IL-17,IL-10 and TGF-β1.
