Effect of Helicobacter pylori infection on gut microbiota in children
10.3760/cma.j.issn.1673-4912.2018.06.013
- VernacularTitle:幽门螺杆菌感染对儿童肠道菌群的影响
- Author:
Wenyu WANG
1
;
Lirong JIANG
;
Zhaohui DENG
;
Ruike ZHAO
;
Xi MO
;
Qing CAO
Author Information
1. 200127,上海交通大学医学院附属儿童医学中心消化科
- Keywords:
Helicobacter pylori;
Gut microbiota;
High throughput sequencing;
Children
- From:
Chinese Pediatric Emergency Medicine
2018;25(6):454-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Helicobacter pylori( HP) on gut microbiota in children by comparing the difference of gastric microbiota between HP-positive and HP-negative individuals. Methods Genome was extracted from excrements of 8 HP-positive cases and 8 HP-negative cases. After genomic extraction,the hypervariable region of 16S rRNA gene were amplified and a small fragment library was constructed,and high-throughput sequencing was carried out, then the data of the lower machine was effectively sequenced by biological information processing. We could seek for the species that have changed significantly due to HP infection by comparing the differences in the composition of intestinal flora between the two groups. Results Compared with HP-negative group,HP-positive group showed less OTUs. The dif-fenece of biodiversity between them was conspicuous. The Caproiciproducens,Enterobacteriaceae,Enterobac-teriales,Blautia-obeum,Esherichia-albertli,human-gut-metagenome and Dorea in HP-positive group were sig-nificantly higher than HP-negative group,while the Bacteroides-uniformis, Bacteroidaceae and Bacteroides in HP-negative group were significantly higher than HP-positive group. Conclusion HP could significantly affect the structure and composition of gut microbiota in children.
