Effects of collagen-bioactive glass-polycaprolactone scaffold on proliferation, migration and differentiation of human dental pulp cells
10.3969/j.issn.2095-4344.0743
- VernacularTitle:胶原-生物活性玻璃-聚己内酯复合支架材料促进人牙髓细胞的增殖、迁移和分化
- Author:
Fu-Qin PAN
1
;
Jian-Hui LIU
;
Yin PANG
;
Yan-Li QIU
;
Jing ZHOU
;
Qi LI
;
Chun-Yi CAI
Author Information
1. 沧州医学高等专科学校口腔系
- From:
Chinese Journal of Tissue Engineering Research
2018;22(14):2168-2173
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Collagen-bioglass-polycaprolacton (COL-BG-PCL) composites have good biocompatibility, mechanical properties and biodegradability that are beneficial to cell adhesion, proliferation and angiogenesis. OBJECTIVE:To study the effect of COL-BG-PCL bioactive scaffold on the proliferation, migration and differentiation of human dental pulp cells (hDPCs). METHODS:hDPCs were isolated and cultured on the COL-BG-PCL bioactive scaffold. MTT, cell scratch test and enzyme-linked immunosorbent assay (ELISA) assay were used to detect the proliferation, migration and differentiation abilities of hDPCs before and at 1, 3, 7, 14, 24 days after inoculation onto the COL-BG-PCL bioactive scaffold. RESULTS AND CONCLUSION:Compared with the cells without inoculation onto the COL-BG-PCL bioactive scaffold, (1) the proliferation ability of the cells cultured on the COL-BG-PCL scaffold was significantly enhanced (P<0.01), and with the prolongation of the inoculation time, the cell proliferation ability was gradually increased; (2) the cell migration ability of the cells cultured on the COL-BG-PCL scaffold was significantly enhanced (P<0.01), and with the prolongation of the inoculation time, the migration ability of the cells cultured on the COL-BG-PCL scaffold was gradually increased; (3) the level of alkaline phosphatase in the supernatant of the cells cultured on the COL-BG-PCL scaffold was significantly increased (P<0.01), and with the prolongation of the inoculation time, the level of alkaline phosphatase in the supernatant was gradually increased. In summary, the COL-BG-PCL scaffold can promote the proliferation, migration and differentiation of hDPCs.
