Effect of cold storage time on cellular viability of umbilical cord mesenchymal stem cells
10.3969/j.issn.2095-4344.0446
- VernacularTitle:治疗用脐带间充质干细胞冷藏保存时间对细胞活性的影响
- Author:
Shan-Shan YAN
1
;
Yi-Jia LI
;
Xi CHEN
;
Li-Jun LIU
Author Information
1. 云南舜喜再生医学工程有限公司
- From:
Chinese Journal of Tissue Engineering Research
2018;22(5):748-753
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: With the in-depth basic research and clinical research on mesenchymal stem cells, mesenchymal stem cells have wide clinical prospects. However, little is reported on the temporary storage conditions and refusion time of mesenchymal stem cells. OBJECTIVE: To explore the effect of cold storage time on the cell mass and viability of umbilical cord mesenchymal stem cells.METHODS: Frozen-thawed and unfrozen umbilical cord mesenchymal stem cells were prepared and stored at 0-4 ℃. The cell viable cell rate, cell doubling time and colony forming efficiency were detected after 0, 6, 12, and 24 hours. RESULTS AND CONCLUSION: Unfrozen cells could maintain the cell biological activity at 0-4 ℃until dead cells appeared with the presence of decreased cell viability at 12 hours after storage. Frozen-thawed cells were unable to be stored at 0-4 ℃ for a long time, and cells began to die and the cell viability was weakened at 6 hours after storage. These findings indicate that umbilical cord mesenchymal stem cells should be injected into patients within 6 or 12 hours after preparation, to ensure the best therapeutic effects. If there is a longer transport distance, it is preferred to use unfrozen mesenchymal stem cells.
