Effect of dietary supplementation of grape skin and seeds on liver fibrosis induced by dimethylnitrosamine in rats.
- Author:
Mi Ok SHIN
1
;
Jeon Ok MOON
Author Information
- Publication Type:Original Article
- Keywords: Grape skin and seeds; hepatoprotective effect; antifibrogenic effect; liver fibrosis; hepatic stellate cell
- MeSH: Actins; Alanine Transaminase; Alkaline Phosphatase; Animals; Anthocyanins; Antineoplastic Agents; Aspartate Aminotransferases; Bilirubin; Collagen; Diet; Dietary Supplements; Dimethylnitrosamine; Fibrosis; Flavonoids; Fruit; Hepatic Stellate Cells; Hydroxyproline; Liver; Liver Cirrhosis; Malondialdehyde; Muscles; Phenol; Rats; Seeds; Serum Albumin; Skin; Vitis; Waste Products
- From:Nutrition Research and Practice 2010;4(5):369-374
- CountryRepublic of Korea
- Language:English
- Abstract: Grape is one of the most popular and widely cultivated fruits in the world. Although grape skin and seeds are waste product of the winery and grape juice industry, these wastes contain large amounts of phytochemicals such as flavonoids, phenolic acids, and anthocyanidins, which play an important role as chemopreventive and anticancer agents. We evaluated efficacies of grape skin and seeds on hepatic injury induced by dimethylnitrosamine (DMN) in rats. Treatment with DMN significantly increased levels of serum alanine transaminase, aspartate transaminase, alkaline phosphatase, and bilirubin. Diet supplementation with grape skin or seeds (10% daily for 4 weeks) prevented these elevations. The grape skin and seeds also restored serum albumin and total protein levels, and reduced the hepatic level of hydroxyproline and malondialdehyde. Furthermore, grape skin and seeds reduced DMN-induced collagen accumulation, as estimated by histological analysis of liver tissue stained with Sirius red. Grape skin and seeds also reduced hepatic stellate cell activation, as assessed by alpha-smooth muscle actin staining. In conclusion, grape skin and seeds exhibited in vivo hepatoprotective and antifibrogenic effects against DMN-induced liver injury, suggesting that grape skin and seeds may be useful in preventing the development of hepatic fibrosis.
