Effect of human umbilical cord mesenchymal stem cells on immune reconstruction of acute lymphoblastic leukemia children undergoing allogeneic hematopoietic stem cell transplantation
10.3969/j.issn.2095-4344.2017.29.015
- VernacularTitle:人脐带间充质干细胞对急性淋巴细胞白血病患儿异基因干细胞移植后免疫重建的影响
- Author:
Jin-Feng XIANG
1
Author Information
1. 南阳市中心医院小儿血液科
- From:
Chinese Journal of Tissue Engineering Research
2017;21(29):4679-4684
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:Human umbilical cord mesenchymal stem cells (hUC-MSCs) can secrete a variety of cytokines and growth factors to support hematopoiesis.They can change the hematopoietic microenvironment,and furthermore,regulate and improve the immune function of the organism.OBJECTIVE:To investigate the effect of hUC-MSCs on immune reconstitution and prognosis in children with acute lymphoblastic leukemia (ALL) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).METHODS:Sixty-four ALL children who had undergo allo-HSCT were enrolled and randomized into non-blood donor allo-HSCT group (n=32) and non-blood donor allo-HSCT+hUC-MSCs group (n=32).The therapeutic effect and complication were observed in the two groups.The changes of immunoglobulins and lymphocyte subpopulations in the two groups were compared before and 1,3 and 6 months after transplantation.RESULTS AND CONCLUSION:Compared with the allo-HSCT group,the time of granulocyte implantation was shorter (P ＜ 0.05),and the incidence of chronic graft versus host disease and cytomegalovirus was lower in the allo-HSCT+ hUC-MSCs group.There were no significant differences in hematopoietic stem cell implantation rate,platelet remodeling rate,cumulative recurrence rate and 1-year survival rate between the two groups (P ＞ 0.05).The levels of serum immunoglobulins IgA,IgG,IgM,IgE and the levels of T lymphocytes (CD4+,CD3+CD4+,CD4+CD8+) and B cells in the peripheral blood of allo-HSCT+hUC-MSCs group were significantly higher than those in the allo-HSCT group at 1,3 and 6 months after transplantation (P ＜ 0.05).In conclusion,the combined use of hUC-MSCs infusion and allo-HSCT can effectively reduce the incidence of chronic graft-versus-host disease,and contribute to granulocyte hematopoietic reconstitution and immune function reconstruction.
