Mechanism of the expression of IL-25 in the lung during asthma
10.7652/jdyxb201804010
- VernacularTitle:IL-25在支气管哮喘中的表达变化及其机制
- Author:
Pei WANG
1
;
Juan HE
;
Li-Hua YANG
;
Pei LU
;
Gang-Qiang WANG
;
Cheng-Hua LI
;
Ruo-Fan YANG
;
Shan-Luan ZHENG
Author Information
1. 西京医院检验科
- Keywords:
asthma;
interleukin 25(IL-25);
transcription factor;
ovalbumin;
bronchial epithelial cell
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2018;39(4):494-497
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular mechanism of interleukin 25 (IL-25)expression in the lung of asthmatic rats.Methods The expressions of IL-25 mRNA and protein in the lungs were detected by Real-time PCR and ELISA,respectively.The levels of IL-25 mRNA and protein were detected by ovalbumin (OVA)in human bronchial epithelial cells.And the transcription factors that regulate IL-2 5 expression were explored through site prediction.Results The expressions of IL-25 mRNA and protein in the lung of OVA-induced asthma rats were significantly increased during animal experiments.Cell experiments showed that OVA could increase the expression of IL-2 5 in human bronchial epithelial cells in a dose-dependent manner,and OVA could upregulate the expression of transcription factor AP1.AP1 was found in the promoter region of IL-25 by site prediction.The AP1 inhibitor (T5224)significantly reduced the expression of IL-25 in OVA-induced human bronchial epithelial cells. Conclusion The molecular mechanism of IL-25 expression induced by OVA in asthma is related to the increase of transcription factor AP1 .
