Affymetrix gene sequencing technique used to analyze the mechanism of pituitary's regulation of CSA model in rats
10.7652/jdyxb201802011
- VernacularTitle:Affymetrix基因芯片测序技术分析垂体对大鼠椎动脉型颈椎病模型的调控机制
- Author:
Min SONG
1
;
Yan-Long GONG
;
Tao LIU
;
Jian-Hong LIU
;
Wan-Tao DONG
;
Xiao-Yu LIU
;
Hong-Yan HOU
Author Information
1. 甘肃中医药大学
- Keywords:
gene chip;
pituitary;
cervical spondylosis of vertebral artery type(CSA);
differential gene;
gene expression profile;
signaling pathway
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2018;39(2):210-215
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze differences in gene expression profile of pituitary tissue in cervical spondylosis of vertebral artery type(CSA)model rats and explore the mechanism of adrenal gland's regulation of CSA.Methods Ten SPF male Wistar rats were randomly divided into model group and blank group.The CSA model was established by compound modeling method.The total RNA was extracted from the pituitary;the gene expression profile was detected by whole gene chip,ontology(GO),and signal pathway analysis.Results Compared with the normal group,the differential genes'expression profile analysis showed that the total number of the differential genes was 321(fold change︱ ＞ 2,P＜0.05),with 203 up-regulated genes and 118 down-regulated genes.A total of 1 294 genes rich in GO function were involved in the regulation of intercellular signal activity and nerve cell function;the stress response to external stimuli;and the regulation of coagulation function,angiogenesis, endometrial system,and cell cycle;There were 145 signal transducers,including adipocytokine signaling pathway, TGF-β signal transduction,AMPK signaling pathway,PPAR signal pathway,Wnt signaling pathway,and MAPK signal transduction pathway.Conclusion The pituitary regulates CSA mainly through the inflammatory stimulation,immune regulation,regulation of vertebral artery function,and endometrial system.
