Effect of MiR-9 on Proliferation and Migration of Human Gastric Cancer Cells
10. 3969/j. issn. 1008-7125. 2018. 06. 005
- VernacularTitle:MiR-9对人胃癌细胞增殖和迁移的影响
- Author:
Wenming LIU
1
;
Yanyun FAN
;
Zhaoxu TIAN
;
Man WAN
;
Fei ZHOU
;
Yiqun HU
Author Information
1. 厦门大学附属中山医院消化内科 厦门大学消化疾病研究所 厦门市消化疾病诊治中心 361004
- Keywords:
Stomach Neoplasms;
MicroRNA-9;
Cell Proliferation;
Cell Migration
- From:
Chinese Journal of Gastroenterology
2018;23(6):342-346
- CountryChina
- Language:Chinese
-
Abstract:
Background:MicroRNA-9 (miR-9)is involved in the modulation of a variety of physiological process such as organ development and self-renewal and multipotential differentiation of cells. Moreover,its aberrant expression is closely associated with several types of malignant tumors. Aims:To investigate the effect of miR-9 on biological behavior of human gastric cancer cells. Methods:Expression of miR-9 in normal human gastric epithelial cell line GES-1 and human gastric cancer cell line BGC-823 and SGC-7901 was detected by real-time PCR. Then the two gastric cancer cell lines were transiently transfected with miR-9 mimic,miR-9 inhibitor and empty vector,respectively;the efficiency of transfection was assessed by real-time PCR. CCK-8 assay,Transwell assay and wound healing assay were used to examine the cell proliferation and migration capacities. Results:The expression level of miR-9 was significantly higher in gastric cancer cells than in normal gastric epithelial cells (P＜0. 05). Compared with cells transfected with empty vector,overexpression of miR-9 occurred in cells transfected with miR-9 mimic and effectively promoted the proliferation and migration of BGC-823 and SGC-7901 cells (P ＜ 0. 05 ). Conversely,transfection with miR-9 inhibitor significantly suppressed the proliferation and migration of gastric cancer cells (P＜0. 05). Conclusions:Up-regulating miR-9 expression promotes the proliferation and migration of gastric cancer cells,which indicates that miR-9 overexpression may be associated with progression of gastric cancer.
