Effect and Underlying Mechanism of Harmine on Proliferation and Apoptosis of Gastric Cancer Cells
10.3969/j.issn.1008-7125.2018.04.006
- VernacularTitle:骆驼蓬碱对胃癌细胞增殖、凋亡的影响及其机制研究
- Author:
Ting ZHANG
1
;
Shiye JIANG
;
Xingxing JIN
;
Wenling ZHANG
;
Na YU
;
Xiaolin LI
;
Guoqin ZHU
;
Yichan ZHOU
;
Yun SHAO
;
Weihao SUN
Author Information
1. 南京医科大学第一附属医院老年消化科 210029
- Keywords:
Harmine;
Stomach Neoplasms;
Cyclooxygenase 2;
Signal Transduction;
Cell Proliferation;
Apoptosis
- From:
Chinese Journal of Gastroenterology
2018;23(4):221-225
- CountryChina
- Language:Chinese
-
Abstract:
Background:Previous study has found that harmine inhibited the proliferation of gastric cancer cells by down-regulating cyclooxygenase-2(COX-2)expression. However,its molecular mechanism is not fully clear. Aims:To investigate the effect of harmine on proliferation and apoptosis of gastric cancer cells,and explore the role of PTEN/Akt/MDM2 signaling pathway in this process. Methods:Human gastric adenocarcinoma cell line SGC-7901 and MKN-45 were treated with harmine at different concentrations(2,4,8,16,32 μg/mL)for 24,48,and 72 hours. The cell proliferation and apoptosis were detected by MTT assay and Hoechst staining,respectively. The expressions of PTEN,COX-2, phosphorylated Akt(p-Akt)and p-MDM2 were measured by Western blotting. Results:Harmine dose- and time-dependently inhibited proliferation and induced apoptosis of SGC-7901 and MKN-45 cells. Also,harmine dose-dependently increased PTEN expression,and inhibited p-Akt,p-MDM2 and COX-2 expressions in SGC-7901 and MKN-45 cells. Conclusions:Harmine may inhibit proliferation and induce apoptosis of gastric cancer cells via down-regulating COX-2 expression through PTEN/Akt/MDM2 signaling pathway.
