Screening and Identification of Differential Proteins of Serum in Individuals Susceptible to Noise Induced Hearing Loss in Tunnel
10.3969/j.issn.1006-7299.2018.02.006
- VernacularTitle:坑道噪声性听力损失易感者血清差异表达蛋白的筛选及鉴定
- Author:
Fujia DUAN
1
,
2
,
3
;
Yanping ZHANG
;
Lina LI
;
Xingwang JIANG
;
Jinwei LIU
;
Lin XIAO
Author Information
1. 解放军309医院耳鼻喉科 北京100091
2. 河北北方学院
3. 浙江省台州医院工作
- Keywords:
Tunnel noise;
Noise induced hearing loss;
Susceptibility;
Proteomics;
Two dimensional electrophoresis
- From:
Journal of Audiology and Speech Pathology
2018;26(2):138-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differential expression of protein in the serum of individuals susceptible to noise induced hearing loss (NIHL) susceptible individuals working in the military tunnel.Methods A total of 40 soldiers from one tunnel construction troop were divided into the susceptible group and the nonsusceptible group.Twenty soldiers were selected for each group.The average age of the susceptible group was 24.79±2.03 years old and their thresholds of the speech and high frequencies were 22.43±8.31 dB HL and 48.55± 11.54 dB HL,respectively.The average age of the nonsusceptible group was 23.67 ± 3.56 years old and their thresholds of the speech and high frequencies were 13.40±4.13 dB HL and 9.40±2.54 dB HL,respectively.Five microliter peripheral venous blood samples were collected from each individual Two-dimensional electrophoresis (2-DE) and MALDI-TOF-MS were used to separate and identify the differentially expressed proteins.Results Thirty-seven protein spots differentially expressing between the NIHL susceptible and nonsusceptible were found after 2 DE.Compared by mascort score,10 differential proteins were harvested.Among these,5 peptides including proteasome subunit alpha-5,complement C4-A,haptoglobin,apolipoprotein A-I and vitronectin were upregulated,and other 5 ones,including Lysozyme C,beta-2 glycoprotein-1,pigment epithelium derived factor,35 kDa trypsin inhibitor heavy chain H and transthyretin were downregulated in NIHL susceptible individuals.The differences were statistically significant(P＜0.05).Conclusion The differentially expressed proteins were closely related to oxidative stress responses in susceptible individuals,including proteasome subunit alpha-5,complement C4A,haptoglobin,apolipoprotein A-I,beta-2 glycoprotein-1,pigment epithelium derived factor,35 kDa trypsin inhibitor heavy chain H and transthyretin.They might participate in the occurrence of NIHL through this way.The proteins harvested from this study were expected to be specific candidate serum NIHL susceptibility biomarkers in blood to help screen susceptible individuals.
