Changes of Auditory Brainstem Response and Expression of Parvalbumin , Calretinin and Calbindin D-28K in the Inferior Colliculus in Rats Following Noise Exposure
10.3969/j.issn.1006-7299.2018.01.017
- VernacularTitle:噪声暴露对大鼠ABR反应阈及下丘中小清蛋白、钙视网膜蛋白及钙结合蛋白表达的影响
- Author:
Tao XU
1
;
Bin LUO
;
Jingwu SUN
;
Congli LIU
;
Chao SONG
Author Information
1. 安徽医科大学附属省立医院耳鼻咽喉头颈外科 合肥 230001
- Keywords:
Noise;
Auditory brainstem response(ABR);
Inferior colliculus;
Parvalbumin;
Calretinin;
CalbindinD-28K
- From:
Journal of Audiology and Speech Pathology
2018;26(1):64-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the possible mechanism of hearing impairment after noise exposure .Methods Adult SD rats (n=14) were monaurally exposed to a loud noise (16 kHz ,one octave band pass (116 dB SPL)) for 1-hour ,or left as non-exposed controls (n=8) .The ABR was measured before and on the 7th day following unilateral noise exposure .All the Animals were euthanased at 7th day following acoustic trauma .We used Western blots to quantify protein levels of PV ,CR and CB in the inferior colliculus .Results The auditory thresholds in 8 , 12 ,16 ,20 ,24 ,and 32 kHz of all the ears exposed to noise showed a significant increase (P<0 .001) .Compared to the sham -exposed controls ,noise-exposed animals had significantly higher levels of PV and CR and lower levels of CB in both the ipsilateral and contralateral IC than controls (P<0 .05) .Respectively ,all the noise-exposed ani-mals had higher levels of PV and CR in the ipsilateral IC than ipsilateral side (P<0 .05) .Conclusion These studies display a significant threshold shift and changes on the expressions of PV ,CR and CB in IC following acoustic trau-ma ,which may be related to noise deafness ,tinnitus and other series of auditory dysfunction .
