The study on cytokines of bronchoalveolar lavage fluid in children with mycoplasma pneumoniae pneumonia
10.11958/20180116
- VernacularTitle:儿童肺炎支原体肺炎肺泡灌洗液中细胞因子分析
- Author:
Wei GUO
1
;
Tong-Qiang ZHANG
;
Yong-Sheng XU
;
Fu-Jun LIU
Author Information
1. 天津市儿童医院呼吸科 300134
- Keywords:
pneumonia,mycoplasma;
inflammation;
bronchoscopy;
interleukins;
child
- From:
Tianjin Medical Journal
2018;46(6):648-651
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the cytokine levels of TNF-α, IFN-γ, IL-2, IL-4, IL-6 and IL-10 in bronchoalveolar lavage fluid (BALF) in children with mycoplasma pneumoniae pneumonia (MPP). Methods Clinical data of 90 patients with MPP (MPP group), who were admitted to Tianjin Children's Hospital from January to December 2017, were collected in this study. The 90 cases were divided into group A1 (<7 days), group A2 (7-14 d) and group A3 (15-30 d) according to the course of disease on admission, with 30 cases in each group. Children who were without MPP and needed bronchoscopy examination or treatment at the same time were used as the control group (B group). The expression levels of cytokines (TNF-α, IFN-γ, IL-2, IL-4, IL-6 and IL-10) were detected by enzyme-linked immunoassay (ELISA). Results The serum and BALF levels of TNF-α, IFN-γ, IL-2, IL-4, IL-6 and IL-10 increased significantly in A group compared with those in B group (P<0.05). The highest levels of cytokines (TNF-α, IFN-γ and IL-2) were found in Group A1. The highest levels of cytokines (IL-4 and IL-6) were found in Group A2. Conclusion The levels of TNF-α, IFN-γ and IL-2 are increased significantly in the disease course of < 7 days. In the disease course of 7 to 14 days, the IL-4 and IL-6 are elevated significantly. Our data suggest that these cytokines may be involved in the pathogenesis of MPP.
