The study of the feasibility of detecting EGFR,KRAS and PIK3CA genes by ROSE cytological slides in non-small cell lung cancer
10.11958/20171502
- VernacularTitle:利用NSCLC患者ROSE细胞学制片进行EGFR、KRAS、PIK3CA基因检测可行性的研究
- Author:
Jing-Jing XU
1
;
Jing FENG
;
Jie CAO
Author Information
1. 天津医科大学总医院呼吸科 300052
- Keywords:
carcinoma;
non-small-cell lung;
mutation;
histocytological preparation techniques;
DNA mutational analysis;
rapid on-site evaluation
- From:
Tianjin Medical Journal
2018;46(3):268-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of EGFR, KRAS and PIK3CA mutation analysis on rapid on-site evaluation (ROSE)cytological slides in patients with non-small cell lung cancer and its clinical value. Methods Seventy-five cases of ROSE cytological slides and paired histological specimens were collected in Tianjin Medical University General Hospital. xTAG70plex liquidchip technology was used to analyze the gene mutations of the samples.Results The KRAS mutation was found in histological specimen but not in ROSE cytological slides in one case. The mutation results were the same in histological specimen and ROSE cytological slides in other cases.The consistent rates of the EGFR mutation and KRAS mutation were 100% and 98.7%,respectively. Conclusion Our study demonstrates that xTAG70plex liquidchip technology can be used for the mutation analysis of EGFR,KRAS and PIK3CA genes in non-small cell lung cancer on ROSE cytological slides.
