Experimental study of lentiviral vector-mediated bFGF gene transfection on rabbit BMSCs
10.11958/20171057
- VernacularTitle:慢病毒载体介导的bFGF基因转染兔BMSCs的实验研究
- Author:
Huai ZHANG
1
;
Wu-Xun PENG
;
Gang LIU
;
Jian ZHANG
;
Fei ZHANG
;
Jian-Bo WANG
;
Yin ZHAO
;
Qing LI
Author Information
1. 贵州医科大学 550004
- Keywords:
stem cells;
fibroblast growth factor 2;
genetic vectors;
transfection;
cell proliferation;
bone marrow stromal cells;
lentiviral vector
- From:
Tianjin Medical Journal
2018;46(2):126-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of lentiviral vector-mediated basic fibroblast growth factor (bFGF) gene transfection on the biological characteristics of rabbit bone marrow stromal cells(BMSCs)under in vitro culture conditions. Methods BMSCs were obtained by density gradient centrifugation and adherence screening. The bFGF gene was transfected into BMSCs by lentiviral vector and divided into bFGF transfection group,empty virus group and untransfected group according to the transfection conditions.After transfection,the morphology,expressions of bFGF mRNA and protein, cell proliferation,cell cycle and alkaline phosphatase(ALP)activity were observed in three groups of cells. Results High density BMSCs were successfully obtained by density gradient centrifugation and adherence screening.After transfection of BMSCs with bFGF gene, the cell morphology showed no significant changes, while the expressions of bFGF mRNA and protein were significantly increased, the cell proliferation curve shifted upward, the proportion of proliferating cells increased,and the activity of ALP was significantly enhanced.There were significant differences between three groups(P<0.05).Conclusion The rabbit bFGF gene is successfully introduced into the BMSCs cultured in vitro by lentiviral vector, and the target gene is stably expressed.The expression of bFGF can promote the proliferation and osteogenic differentiation of BMSCs.
