The expression and role of LncRNA MEG3 in human glioma cells
10.11904/j.issn.1002-3070.2018.03.005
- VernacularTitle:长链非编码RNA MEG3在人胶质瘤细胞中的表达及作用研究
- Author:
Ningning FAN
1
;
Jingshu GENG
Author Information
1. 哈尔滨医科大学附属肿瘤医院病理科 哈尔滨 150081
- Keywords:
LncRNA;
MicroRNA;
Glioma;
Apoptosis;
Molecular mechanism
- From:
Practical Oncology Journal
2018;32(3):219-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to illuminate effects of LncRNA MEG3 on the viability and apoptosis of hu-man glioma cells as well as the mechanisms involved. Methods The expressions of MEG3 and microRNA-21(miR-21) in normal human astrocytes cell line(NHAs)and human glioma cell line(U87)was detected by Real-Time quantitative polymerase chain reac-tion(RT-qPCR). U87 cells was transfected with MEG3 plasmid alone or transfected with both MEG3 plasmid and miR-21 mimic, cell viability was detected by CCK-8 assay,cell apoptosis was detected by TUNNEL,and the expression of Bcl-2/Bax and Cleaved caspase-3 in cells was detected by Western blot. Results Compared with NHAs,the expression of MEG3 in U87 was significantly decreased and the expression of miR-21 was significantly increased. Overexpression of MEG3 significantly decreased the expression of miR-21 in U87 cells,inhibited the viability of U87 cells,decreased the level of Bcl-2/Bax,increased the content of Cleaved-caspase-3 to promote apoptosis;Co-overexpression of MEG3 and miR-21 significantly increased the viability of U87 cells,elevated Bcl-2/Bax levels,and decreased the expression of Cleaved caspase-3 to inhibit apoptosis. Conclusion Overexpression of MEG3 reduces the viability and promotes apoptosis of glioma cells by inhibiting miR-21.
