Effect of Wogonin on osteosarcoma cells and its mechanism based on a PI3K/Akt signaling pathway
10.11904/j.issn.1002-3070.2018.03.004
- VernacularTitle:基于PI3K/Akt信号通路的汉黄芩素抗骨肉瘤作用机制研究
- Author:
Dapeng DUAN
1
;
Shanshan LIU
;
Weiwei LI
;
Min FENG
;
Le JI
;
Jing QIN
Author Information
1. 陕西省人民医院 西安 710068
- Keywords:
Wogonin;
Osteosarcoma;
Proliferation;
Apoptosis;
PI3K/Akt pathway
- From:
Practical Oncology Journal
2018;32(3):214-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aims of this study were to explore effects of Wogonin on proliferation,apoptosis and the PI3K/Akt signaling pathway in human osteosarcoma MG-63 cells. Methods MG-63 cells were treated with different concentrations of Wogo-nin(0,50,100 and 200 μmol/L)for 24,48 and 72 h,MTT assay was used to determine the cell proliferation,and the rates of apoptosis of MG-63 cells were assessed by the flow cytometry;Cell scratch assay was used to detect cell migration and TEM to detect morpho-logical changes of MG-63 cells;Western blot were employed to examine the expression of COX-2,Caspase-3 and P-Akt protein in MG-63 cells. Results Wogonin significantly inhibited proliferation of MG-63 cells in a dose-or time-dependent manner,and this effect was positively correlated with drug concentration and duration of action(P<0. 05). The apoptotic rate of MG-63 cells was positively correlated to the concentrations of Wogonin. Their differences were statistically significant(P<0. 05). Wogonin also signifi-cantly inhibited the migration of MG-63 cells in a dose-dependent manner(P<0. 05). Wogonin significantly down-regulated the levels of COX-2 and p-Akt protein expression and significantly up-regulated the level of caspase-3 protein expression in MG-63 cells when compared to the negative control group(P<0. 05). Conclusion Wogonin can inhibit the proliferation of MG-63 cells and promote apoptosis,which may be related to down-regulation of PI3K/Akt signaling pathway.
