Effects of Wnt pathway inhibitor ETC-159 on proliferation and migration of OSCC cells
10.11904/j.issn.1002-3070.2018.02.002
- VernacularTitle:Wnt通路抑制剂ETC-159处理对口腔鳞癌细胞增殖迁移的影响
- Author:
Yanjun DUAN
1
;
Wei XU
;
Wenjie CHEN
;
Fengjuan WANG
Author Information
1. 襄阳市中心医院
- Keywords:
Oral squamous cell carcinoma;
ETC-159;
Wnt pathway;
Proliferation;
Migration
- From:
Practical Oncology Journal
2018;32(2):103-106
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to investigate the effect of Wnt pathway inhibitor-ETC-159 on pro-liferation and migration of oral squamous cell carcinoma(SCC-15)cells and to explore its mechanism.Methods SCC-15 cells were treated with DMSO and ETC-159 for 12 h or 24 h.Cell proliferation was detected by CCK8 kit.Transwell assay was used to de-tect the ability of cell migration.Western blotting was used to detect cell migration related to proteins i.e.Wnt3a and β-catenin,pro-liferation and migration related proteins i.e.c-Myc,cyclin D1,CD146.Results After treated with ETC-159 for 12 or 24 h,the proliferation,migration and expression of Wnt3a,β-catenin,c-Myc,cyclin D1 and CD146 in SCC-15 cells were significantly de-creased when compared to the DMSO group(P<0.05).Conclusion Wnt signaling pathway inhibitor-ETC-159 can inhibit the proliferation and migration of SCC-15 cells by decreased levels of c-Myc,cyclin D1 and CD146.
