Telomere length in cumulus cells as a potential biomarker of embryo quality and pregnancy rate
10.3969/j.issn.1006-5725.2018.12.024
- VernacularTitle:颗粒细胞端粒长度预测胚胎发育潜能及妊娠率的临床研究
- Author:
Nan LI
1
;
Ni TANG
;
Lihong WEI
;
Xinlin LI
;
Zhong LIN
Author Information
1. 柳州市妇幼保健院生殖医学中心
- Keywords:
intracytoplasmic sperm injection(ICSI);
cumulus cell;
telomere length;
pregnancy rate
- From:
The Journal of Practical Medicine
2018;34(12):2019-2022,2026
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship of relative telomere length in cumulus cells(CCs) with oocytes at different mature stages and the outcome of intracytoplasmic sperm injection and embryo transfer (ICSI-ET). Methods Oocyte-cumulus complex samples were collected from 92 patients undergoing ICSI-ET and patients were divided into group A including 55 women≤35 years and group B 37 women>35 years. The embryonic development ability and the result of clinical pregnancy were recorded in different groups. DNA was extracted from CCs and assessed for telomere length by real-time quantitative PCR. Results In ICSI-ET,the relative telomere length of CCs gradually shortened with the age.There was a significantly longer telomere length of CCs in the preg-nant subgroup than that in the non-pregnant subgroup in the two groups(P < 0.05). Conclusion The telomere length of CCs may be associated with the pregnancy outcome in ICSI-ET.
