Expressions of β-catenin and LEF1 in acute leukemia and their correlation
10.3969/j.issn.1006-5725.2018.12.016
- VernacularTitle:急性白血病β-catenin与LEF1基因表达的相关性
- Author:
Wei WANG
1
;
Wenxin LIU
;
Yongbin HUANG
;
Yunguang HONG
;
Zhigang YANG
Author Information
1. 广东医科大学附属医院血液病研究室
- Keywords:
Acute leukemia;
β-catenin;
LEF1;
Correlation
- From:
The Journal of Practical Medicine
2018;34(12):1986-1989
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of β-catenin and lymphoid enhancer factor 1(LEF1) mRNA in acute leukemia(AL)and their correlation. Methods Real-time PCR was used to examine the expres-sions of β-catenin and LEF1 mRNA in 62 de novo acute myeloid leukemia(AML)patients,23 de novo acute lymphoblastic leukemia(ALL)patients,20 controls and 4 kinds of cell lines:K562,HL-60,THP-1 and Raji. Results The expression level of β-catenin in AML group was significantly higher compared with the control group,but LEF1 mRNA expression in AML group was significantly lower than that in the control group (P <0.05). The expression levels of β-catenin and LEF1 mRNA were all significantly higher in ALL group compared with the control group (P < 0.05). There were significantly positive correlations between β-catenin and LEF1 mRNA expressions both in AML and in ALL groups(P<0.05). Among the three AML cell lines,THP-1 cell had the highest β-catenin mRNA expression and the lowest LEF1 mRNA expression,and the expression tendencies of these two genes in THP-1 cell line were in accordance with those in AML patients. Conclusions Activation of Wnt/β-catenin pathway happends in AL patients and β-catenin/LEF1 may play a role in leukemogenensis of ALL.
