Effect of recombinant human erythropoietin on the proliferation of neural stem cells derived from central canal of adult rat spinal cord
10.3969/j.issn.1006-5725.2018.12.013
- VernacularTitle:重组人促红细胞生成素对成年大鼠脊髓中央管膜下神经干细胞增殖能力的影响
- Author:
Xiaoqi ZHOU
1
;
Bo WU
;
Xinping YAN
;
Meisong ZHU
;
Yankai PENG
;
Zhong. CHEN
Author Information
1. 南方医科大学珠江医院脊柱外科
- Keywords:
erythropoietin;
adult rats;
spinal cord;
neural stem cells;
proliferation
- From:
The Journal of Practical Medicine
2018;34(12):1973-1977
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of erythropoietin(EPO)on the proliferation of neural stem cells(NSCs)derived from central canal of adult rat spinal cord in vitro ,so as to provide a theoretical basis for clinical treatment of spinal cord injury by autotransplanting or allograft transplanting of adult spinal cord NSCs. Method NSCs were isolated from the central canal of the adult rats spinal cord by microsurgical method,and Nestin(nestin)and Sox2 immunofluorescence stain were used to identify the cells. After cells were treated with different dose of EPO,5,10,20 and 40 U/mL,respectively,the optical treatment concentration and time were determined by CCK8 assay. The effect of EPO on the cell count and the expression of Cyclin D1 in NSCs were detected at the treatment time 96 h. Result The NSCs derived from the central canal of adult SD rats spinal cord could stably express protein Nestin and transcription factor Sox2. As the results of CCK8 test,cell counts and real-time quantitative PCR showed the optimal treatment of concentration and time maybe 20 U/mL and 96 h. Conclusions This study shows that EPO can promote the proliferation of NSCs derived from central canal of adult rat spinal cord,and the optimal treatment of concentration and time for proliferation might be 20 U/mL and 96 h.
