Effects of sevoflurane on HIF-1α/EMT pathway activity and invasion of lung cancer in rats undergoing one lung ventilation
10.3969/j.issn.1006-5725.2018.12.012
- VernacularTitle:七氟醚对单肺通气大鼠肺癌HIF-1α/EMT通路活性及侵袭力的影响
- Author:
Yanfeng XIONG
1
;
Xiaohong LAI
;
Hua LIANG
;
Tao ZHANG
;
Qiaoling ZHOU
;
Chengxiang YANG
Author Information
1. 佛山市第一人民医院麻醉科
- Keywords:
lung cancer;
one lung ventilation;
sevoflurane;
HIF-1α;
EMT
- From:
The Journal of Practical Medicine
2018;34(12):1970-1972
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sevoflurane on HIF-1α/epithelial mesenchymal transition (EMT)pathway activity and invasion of lung cancer in rats undergoing one lung ventilation (OLV). Methods Lung cancer model of SD rats was established. Rats were randomly divided into 4 groups:group control(group C), group two lungs ventilation(TLV)(group T),group one lungs ventilation(group O),and group sevoflurane +one lungs ventilation(group SO). Two lung ventilation was performed after endotracheal intubation for 2.5 h in group T. OLV was performed after endotracheal intubation for 2 h in group O and SO. The end-expiratory concentration of sevoflurane of rats in group SO was maintained 2.6% during OLV period. Left lung cancer tissues were harvested at 0.5 h of TLV. The protein levels of HIF-1α,Vimentin and Fibronectin in lung cancer were determined by Western blot. The mRNA levels of MMP-2 and MMP-9 in lung cancer were evaluated by RT-PCR. Results The expres-sions of HIF-1α,Vimentin,Fibronectin,MMP-2,and MMP-9 in group O and group SO were significantly higher than those in group C and group T(P<0.05). The expressions of HIF-1α,Vimentin,Fibronectin,MMP-2,and MMP-9 were decreased significantly in group SO as compared with group O(P<0.05). Conclusion Sevoflurane inhibits the elevation of HIF-1α/EMT pathway activity and invasion ability induced by OLV.
