Pirfenidone inhibits TGF-β1-induced fibration in rat corneal stromal cells
10.3969/j.issn.1006-5725.2018.12.009
- VernacularTitle:吡非尼酮抑制转化生长因子β1诱导的大鼠角膜基质细胞纤维化
- Author:
Gongfa WU
1
;
Lizhen QIU
;
Qiting HUANG
;
Yujun LIU
;
Yuting ZENG
;
Junjie CHEN
Author Information
1. 中山大学孙逸仙纪念医院
- Keywords:
pirfenidone;
corneal stromal cell;
TGF-β1;
fibrosis
- From:
The Journal of Practical Medicine
2018;34(12):1955-1958
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of pirfenidone (PFD) on the transformation of rat corneal stromal cells into fibroblasts in vitro and further explore the anti-fibrotic effect of PFD. Methods The corneal stromal cells from SD rat was isolated and cultured ,and was determined by vimentin stain. The experiment was divided into control group(DMEM+10%FBS),TGF-β1 group(2 ng/mL TGF-β1+DMEM+10%FBS)and PFD group(1 mg/mL PFD+ 2 ng/mL TGF-β1+DMEM+ 10%FBS). Cell proliferation was detected by CCK-8 assay. Collagen Ⅰ,Collagen Ⅲ,Keratocan and CD99 expression were detected by Western blot. Results Compared with control group and TGF-β1 group,the cell proliferation were significantly decreased in PFD group(P<0.05). Western blot showed that PFD can up-regulated Collagen Ⅰand Keratocan but down-regulated Collagen ⅢCD90 expression(P < 0.05). The ratio of Collagen Ⅲ/Collagen Ⅰ in PFD group was lowest in all groups(P < 0.05). Conclusion PFD can resistant fibration in corneal stromal cells may through the inhibition of TGF-β ,which affect the collagen synthesis and Keratocan,CD90 expression.
