Suppression effect of secreting-trail adenovirus on the prostate cancer PC-3 cells in the tumor bearing mice
10.3969/j.issn.1006-5725.2018.12.008
- VernacularTitle:重组分泌型肿瘤坏死因子相关凋亡诱导配体腺病毒对PC-3细胞荷瘤鼠瘤体的生长抑制
- Author:
Lei GAO
1
;
Tiejun PAN
;
Ming XIE
;
Yuan JIANG
;
Xi YU
;
Xiang LI
Author Information
1. 中国人民解放军武汉总医院泌尿外科
- Keywords:
prostate cancer;
tumor bearing mice;
cell apoptosis;
secreting-trail adenoviral vector
- From:
The Journal of Practical Medicine
2018;34(12):1951-1954,1958
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the suppression effect of secreting-trail adenovirus on the prostate cancer PC-3 cells in the tumor bearing mice. Methods Apoptosis of PC-3 cells was measured by DAPI staining in vitro . Invasion of PC-3 cells was measured by transwell chamber assay. The tumor bearing mice were prepared and used to test the inhibition rates in different groups. The protein expression of TRAIL in different groups tumor bearing mice was determined by immunohistochemistry. The variation of cell apoptosis in different groups of tumor bearing mice was detected by TUNEL assay. Results DAPI staining showed that the apoptosis level in Ad-sTRAIL group was significantly higher than that in the control group and that in the Ad-LacZ group ,respectively. The number of PC-3 cells invading the inferior chamber in the Ad-sTRAIL group(24.8 ± 3.70) was significantly decreased compared with that in the control group(47.6 ± 4.28,q=10.68,P<0.01)and that in the Ad-LacZ group (49.6 ± 4.39,q=11.61,P<0.01). Growth inhibition assays in vivo showed the inhibition rate of Ad-sTRAIL was 3 d 8.15%,6 d 11.55%,9 d 17.23%,12 d 20.05%,15 d 27.18%,18 d 34.27%,and 21 d 33.08%,respective-ly. Immunohistochemistry results showed that the expression level of TRAIL in tumors of Ad-sTRAIL group tumor-bearing mice was significantly higher than that in the control group and that in the Ad-LacZ group. TUNEL assay showed that the apoptosis level of tumor bearing mice tumor cells in the Ad-sTRAIL group was significantly higher than that in the control group and that in the Ad-LacZ group. Conclusion The secreting-trail adenoviral vector could inhibit the growth of tumor bearing mice tumor cells,furthermore it could induce the apoptosis of PC-3 cells in the tumor bearing mice.
