Effects of nifedipine on oxidative stress causing by iron-overload in HK-2 cells
10.3969/j.issn.1006-5725.2018.12.005
- VernacularTitle:硝苯吡啶在铁超载HK-2细胞氧化应激中的作用及机制
- Author:
Xiaomei LÜ
1
;
Bing RAN
;
Mao YANG
;
Xuesen LI
;
Yan LIN
Author Information
1. 西南医科大学生理学教研室
- Keywords:
iron-overload;
nifedipine;
HK-2;
oxidative Stress
- From:
The Journal of Practical Medicine
2018;34(12):1938-1941
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and the mechanism of nifedipine on oxidative stress trip-ping by iron-overload of HK-2 cells. Methods The cells were divided into 4 groups,blank group,iron overload group,nifedipine group and FAC with nifedipine co-treatment group. Cells were treated with FAC or/and nifedipine for 24 hours,and then malonaldehyde (MDA) content,superoxide dismutase (T-SOD) activity,glutathione (GSH) content,intracellular iron content and expression of DMT1 and FPN1 protein were evaluated. Results Compared to the iron overload group,both nifedipine treatment and co-treatment decreased the content of MDA (P < 0.05),increased the activity of SOD(P < 0.05),increased the content of GSH(P < 0.05),reduced the intracellular iron content(P<0.05),increased the expression of DMT1(P<0.05),and increased the expression of FPN1(P < 0.05). Conclusion Nifedipine plays a protective role against oxidative stress induced by iron-overload in HK-2 cells,and it is related to promote DMT1 and FPN1 protein expression and reduce intracellular iron content.
