Establishment of transgenic mice model with overexpression of neuritin in bone marrow
10.3969/j.issn.1006-5725.2018.10.011
- VernacularTitle:骨髓特异性高表达neuritin转基因小鼠的构建
- Author:
Yuanzhi LI
1
;
Jiyin ZHOU
;
Zuo ZHANG
;
Hejiao LI
;
Yilan HUANG
Author Information
1. 西南医科大学药学院 四川泸州 646000
- Keywords:
neuritin gene;
transgenic mice;
lyz-Cre;
cre-loxp recombination system;
genotyping
- From:
The Journal of Practical Medicine
2018;34(10):1624-1627,1632
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish transgenic mice model with over expression of neuritin in bone mar-row,for the further study on the function of neuritin protein in the treatment of peripheral neuropathy. Methods Two pairs of transgenic mice(loxp-stop-loxp-neuritin and lyz-Cre/+)were fed and propagated,the DNA from the mice tails extracted and the genotype of transgenic mice identified by PCR. The wild type mice with B6 were as-signed as the controls,and the immunofluorescence was used to detect the accuracy of the neuritinloxp/+ _lyz -Cre/+. Results The two trensgenetic homozygous mice had the ability to reproduce,and the hybrid offsprings were neuritinloxp/+_lyz-Cre/+,neuritinloxp/-_lyz-Cre/+,neuritinloxp/+_lyz-Cre/-,neuritinloxp/-_lyz-Cre/-. The re-sults were met with the Mendel′s law. The results of immunofluorescence showed that the expression of neuritin of neuritinloxp/+_lyz-Cre/+ mice in bone marrow was significantly higher than the wild type mice(P < 0.05). Con-clusion The PCR method is of high reliability for identification of sub pus genotype and the female neuritinloxp/+mice mating with the male lyz-Cre/+ ones is an effective way for obtaining the neuritinloxp/+_lyz-Cre/+ mice.
