Study the mechanism of tantalum particles on the proliferation of osteoblasts
10.3969/j.issn.1006-5725.2018.10.010
- VernacularTitle:钽颗粒影响成骨细胞增殖的机制研究
- Author:
Chengrong KANG
1
;
Liang LI
;
Zhangwei LI
;
Zhechong ZHOU
;
Qianbing ZHOU
;
Xuan PAN
Author Information
1. 广东药科大学附属第一医院口腔科 广州 510080
- Keywords:
tantalum particles;
osteoblast;
proliferation;
autophagy
- From:
The Journal of Practical Medicine
2018;34(10):1618-1623
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tantalum particles on the proliferation of osteoblasts and explore its mechanism. Methods Mouse osteoblasts MC3T3-E1 were co-cultured with micro-tantalum particles (micro-Ta)and Nano-tantalum particles(nano-Ta)of different concentrations respectively. CCK-8 assay was used to measure the cell viability at 6,12,24 and 48 h. According to the result of CCK-8 the group with the most prolif-erative effect was screened and the level of autophagy was detected by using Western blot,laser confocal microsco-py and transmission electron microscopy(SEM). Finally,to verify the role of autophagy in pro-proliferation effect of nano-Ta,the OD value was measured repeatedly in combination with autophagy inducer and inhibitor. Results 100 ng/mL micro-Ta treated groups had obvious proliferative effect but autophagy was not detected. 20 μg/mL nano-Ta treated groups had obvious proliferative effect and autophagy was detected. CCK-8 assay revealed that autophagy inhibitor can significantly inhibited cell proliferation of nano-Ta treated group. Conclusion Nano-Ta could pro-mote cell proliferation by inducing autophagy,and micro-Ta may promote osteoblast proliferation through other non-autophagy pathway.
