Correlations of mutations of IDH1,IDH2,p53 gene and Ki-67 protein expression in gliomas with the clini cal features of pathological grading
10.3969/j.issn.1006-5725.2018.09.012
- VernacularTitle:脑胶质瘤分子标志物IDH1、IDH2、p53基因突变和Ki-67蛋白表达与病理分级临床特征的关系
- Author:
Yi SUN
1
;
Hui LI
;
Yi WU
;
Jian LI
;
Longshu SHEN
;
Yaohui WANG
;
Jianrong WANG
;
Yifen ZHANG
Author Information
1. 南京中医药大学附属江苏省中医院病理科/国家中医药管理局分子生物学三级实验室 南京 210029
- Keywords:
glioma;
mutation;
IDH1;
IDH2;
p53;
Ki-67
- From:
The Journal of Practical Medicine
2018;34(9):1455-1459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mutations of IDH1,IDH2,p53 gene,and Ki-67 protein expression in different grade of gliomas and identify the association with its clinical relevance. Methods The mutations of IDH1,IDH2 and p53 gene were detected by direct DNA sequencing,and protein expression of Ki-67 was analyzed by immunohistochemistry. The correlations between gender,age,tumor site,differentiation degree and pathological type of patients were analyzed. Results R132H mutation of IDH1 gene was detected in 32.6% samples (14/46 cases),of which the proportion of WHO classification grade Ⅱ was 40.0%,and grade Ⅲ was 58.3%. IDH1 mutations were shown correlated with age,pathology level Ⅱ-Ⅲ,and Ki-67 low expression. p53 mutations were detected in 4 glioblastomas,with mutations located at exon 7,8. IDH1 gene mutation was negatively correlated with Ki-67 expression. Conclusions The proportion of IDH1 gene mutation in different pathological types of gliomas is different,which is the highest in gradeⅡ~Ⅲ. It is suggested that the subtypes should be listed independently by routine tests. Mutations in p53 gene are more common in primary glioblastomas and may be associated with adverse outcomes. The combined detection of DH1,p53 and Ki-67 is conducive to the diagnosis and prognosis of glioma.
