Changes in inflammatory factors and protein expression in sulfur mustard(1LD50)-induced pulmonary in- jury in rats

VernacularTitle:等毒性剂量芥子气诱导大鼠肺损伤炎性因子和蛋白表达变化
Author: Dan YU ¹ ; Yuxu ZHONG ; Yuan LI ; Jianwei XIE ; Xiaoji ZHU
Author Information

1. 潍坊医学院研究生部山东淮坊261053
Keywords: sulfur mustard; equal toxicity dose; pulmonary injury; inflammatory factor
From: The Journal of Practical Medicine 2018;34(9):1450-1454
CountryChina
Language:Chinese
Abstract: Objective To establish an animal model of SM by equal toxicity dose(1LD50)-induced acute pulmonary injury in rats and compare the differences of inflammatory factor and protein expression. Methods Rats were randomly divided into five groups. ELISA and immunohistochemical methods were measured. Results Serum INF-γ and IL-23 levels in the intraperitoneal SM group were increased compared with the tracheal SM group;there were also significant differences in serum IL-4 levels between the two groups. In the alveolar septum , the positive expression ratios of NF-Kβ1,NF-Kβp65,ERK,JNK,and p38MAPK in the intraperitoneal SM group were increased compared with the tracheal SM group. Conclusion Using SM (1LD50),There are significantly higher serum inflammatory factor levels and protein-related expression in the alveolar septum of rats intraperitoneally injected with SM compared with those administered SM by intratracheal instillation. The results suggest that pulmonary inflammatory reactions associated with SM are dependent on the route of exposure.