Protective effect of hyperbaric oxygen preconditioning on rat syngeneic kidney transplantation
10.3969/j.issn.1006-5725.2018.08.009
- VernacularTitle:高压氧预处理在大鼠同系异体肾移植中的保护作用
- Author:
Xiaoxiang CHEN
1
;
Hui ZHANG
;
Lu YANG
;
Wei XIONG
;
Xiongqing HUANG
Author Information
1. 中山大学附属第一医院麻醉科 广州510080
- Keywords:
kidney transplantation;
hyperbaric oxygen preconditioning;
heme oxygenase-1
- From:
The Journal of Practical Medicine
2018;34(8):1258-1261
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hyperbaric oxygen preconditioning on syngeneic kidney transplantation in rats and to explore the potential mechanism. Methods Forty male SD rats were randomly divid-ed into control group(Sham group),kidney transplantation group(KTx group)and hyperbaric oxygen precondi-tioning group(HBO+KTx group). Kidney transplantation was performed 24 h after hyperbaric preconditioning. Rats were sacrificed 24 h after the transplantation.Serum creatinine(Scr)and blood urea nitrogen(BUN)were de-tected.The level of superoxide dismutase(SOD),malondialdehyde(MDA)and heme oxygenase-1(HO-1)in the graft was examined. Kidney pathological examination was performed after renal tissue fixed. Results Compared with the KTx group,HBO + KTx group showed lower Scr and BUN level. The MDA level was decreased and the SOD activity was increased significantly after hyperbaric oxygen preconditioning in HBO+KTx group.Renal tubu-lar necrosis was significantly reduced in HBO+KTx group.HO-1 level in HBO+KTx group was significantly high-er than that of KTx group. Conclusion Hyperbaric oxygen preconditioning can reduce lipid peroxidation,en-hance antioxidant enzyme activity and improve the level of HO-1 in graft tissue,thus attenuating kidney injury.
