Analysis of endogenous antioxidant system and oxidative stress level in patients with diabetic cardiomyopa-thy
10.3969/j.issn.1006-5725.2018.08.003
- VernacularTitle:糖尿病心肌病患者内源性抗氧化体系及氧化应激水平
- Author:
Xiaodan QI
1
;
Lingfeng CHANG
;
Haitao YU
;
Ying LIU
;
Yong GAO
;
Shudong LIN
;
Xiaoguang YAN
;
Dongxia XU
;
Chunjing ZHANG
Author Information
1. 齐齐哈尔医学院 161006黑龙江齐齐哈尔
- Keywords:
diabetic cardiomyopathy;
antioxidant system;
oxidative stress
- From:
The Journal of Practical Medicine
2018;34(8):1231-1234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the endogenous carnosine,glutathione system and oxidative stress level in serum of patients with diabetic cardiomyopathy(DCM),and the potential relationships among them.Meth-ods The serum of 102 healthy people,96 patients with type 2 diabetes mellitus and 74 patients with diabetic car-diomyopathy in the third affiliated hospital of QiQihar Medical University were enrolled.Carnosine content was mea-sured by ELISA.T-GSH,GSH and GSSG were detected by using micro-enzyme labeling assay.GSH-Px,GST and GRAC were detected by using colorimetry. The content of NO was detected by nitrate reduction method. The con-tent of H2O2was detected by using molybdic acid coloring method. The activities of NOS and CAT was determined by colorimetry. Results Compared with the healthy control group,the average level of human serum carnosine, GSH content,GRAC,GSH/GSSG ratio,GST activity and T-SH content in the DM and DCM group were signifi-cantly reduced(P < 0.05,respectively). NO content,H2O2and iNOS activity were increased,but CAT activity was decreased in DCM group.Conclusion Decreases of levels of serum carnosine and glutathione(GSH)and the imbalance of redox state were observed in patients with diabetic cardiomyopathy,which may promote the occur-rence and development of the diabetic cardiomyopathy.
