Effects of rhPTH(1-34)treatment for glucocorticoid-induced osteoporosis
10.3969/j.issn.1006-5725.2018.06.017
- VernacularTitle:rhPTH(1-34)对糖皮质激素性骨质疏松症的防治作用
- Author:
Ping SUN
1
;
Qiangqiang XING
;
Guoju HONG
;
Guozhu YANG
;
Nan LIU
;
Weishan SUN
;
Lingping HU
;
Weimin DENG
;
Chenghong MA
Author Information
1. 广东药科大学附属第一医院骨内科 广州510080
- Keywords:
GIOP;
rhPTH(1-34);
β-catenin;
Wnt10b
- From:
The Journal of Practical Medicine
2018;34(6):941-945
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the GIOP model and extract BMSCs from the rat model.We aim to in-vesitigatethe effect ofrhPTH(1-34)for inhibiting β-catenin ubiquitination when combining with Micro-CT and bio-logical technology.We also investigate the influence of rhPTH(1-34)on the GIOP.Methods Female SPF emale rats wererandomly divided into normal control group,methylprednisolone group(model group),methylpredniso-lone+saline group(blankcontrol group)and methylprednisolone+rhPTH(1-34)group(test group). The proximal femoral cancellous bone was examined by Micro-CTand histopathological Staining. The expression of Wnt10b and β-catenin protein were detected. By comparing with inducedBMP-2,BMSCs were treated withrhPTH(1-34)and stained with ALP and alizarin red.Results(1)In Micro-CT,BV/TV,Tb.Th and Tb/N decreased,whereas Tb/sp increased in the test group comparedwith model group(P<0.05).ROI three-dimensional reconstruction of trabecu-lar bone in test group showed local bone repair;(2)Wnt10b and β-cateninexpression increased in the test group compared with the model model(P<0.05),indicating that rhPTH(1-34)can enhance the transcriptional activity of β-catenin(P<0.05)and promote the expression of Wnt10b andβ-catenin(P<0.05).Conclusion The inter-vention with rhPTH(1-34)can prevent GIOP by regulating the Wnt/β-catenin signaling pathway and inhibiting GIOP progress,which can improve the microstructure of bone.
