The experimental study on repair of rat femoral condyle defect with peripheral blood mesenchymal stem cells combined with absorbable gelatin sponge-self assembling peptide
10.3969/j.issn.1006-5725.2018.06.013
- VernacularTitle:外周血间充质干细胞联合可吸收明胶海绵-自聚合肽修复大鼠股骨髁缺损
- Author:
Zhiming ZHANG
1
;
Chun LIU
;
Guofeng WU
;
Yanlin CAO
;
Yanglei JIN
;
Yihang WANG
;
Jiasong GUO
;
Lixin ZHU
Author Information
1. 南方医科大学第二附属医院(珠江医院)骨科中心 广州510280
- Keywords:
peripheral blood mesenchymal stem cells;
bone defect;
absorbable gelatin sponge;
self assembling peptide;
tissue engineering
- From:
The Journal of Practical Medicine
2018;34(6):924-928,932
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to explore the effect of peripheral blood mesenchymal stem cells combined with porous absorbable gelatin sponge/self assembling peptide composite scaffolds on SD rat femoral con-dyle bone defect reconstruction and provide a new strategy for the repair of bone defects. Methods 30 female SD rats,8W age,were randomly divided into 3 groups,10 every group.The group A was blank control group,group B was porous absorbable gelatin sponge/self assembling peptide composite scaffold group,and group C was periph-eral blood mesenchymal stem cells combined with porous absorbable gelatin sponge/self assembling peptide compos-ite scaffold group. The effect of osteogenesis was observed by paraffin section,hematoxylin eosin staining,X-ray examination,and Micro-CT scanning in 3 dimensional reconstruction of femoral condyle defect. Results Imaging examination showed that the experimental group had better osteogenesis effect. Histological examination showed that a lot of new bone tissue was found in group C,while only a small amount of new bone was found in the group of A and B. Conclusions The experiment shows that peripheral blood mesenchymal stem cells as the seed cells for tissue engineering,combined with porous absorbable gelatin sponge-self assembling peptide has better ability to repair bone defects,and has good application prospect,which is worthy of further research.
