Cholinergic signal regulates neural stem cell differentiation in perilesional zone after ischemic stroke
10.3969/j.issn.1006-5725.2018.06.001
- VernacularTitle:胆碱能信号调节脑梗死后自体神经干细胞分化
- Author:
Jianping WANG
1
;
Xianliang LIU
;
Xiaojie FU
;
Di ZHANG
;
Zhengfang LU
;
Chunmao YIN
;
Junji KE
;
Jiang MAN
;
Sijia LI
Author Information
1. 郑州大学脑血管病研究所
- Keywords:
brain infarction;
cholinergic signal;
neural stem cell;
neural differentiation
- From:
The Journal of Practical Medicine
2018;34(6):877-881,884
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of cholinergic signal on neural stem cell(NSC)differenti-ation in peri-infarction region after ischemic stroke. Methods Mice were randomly assigned into sham + vehicle group,middle cerebral artery occlusion(MCAO)+ vehicle group,MCAO + donepezil group and MCAO + atro-pine group(n = 25). MCAO was induced by thread-occlusion method. Modified neurological severity score (mNSS)was used to evaluate neurological function recovery,and the brain water content was measured by dry-wet weight method. NeuN/5-bromodeoxyuridine(BrdU),CNPase/BrdU,GFAP/BrdU double-labeled cells were tested by immunofluorescence. Results Brain water content of MCAO + vehicle group was significantly higher than that of sham operation group(P < 0.05). Donepezil-treated MCAO mice had lower neurologic deficit scores and brain water content than of MCAO + vehicle group(P < 0.05). On day 14 and day 28 after MCAO,the NeuN/BrdU, CNPase/BrdU and GFAP/BrdU immune-positive cells of MCAO + vehicle group were markedly increased as com-pared with that of sham+vehicle group(P<0.05).Compared with that of MCAO+vehicle group,the number of NeuN/BrdU-positive cells,CNPase/BrdU-positive cells and GFAP/BrdU-positive cells was higher in MCAO+done-pezil group,and the number of NeuN/BrdU-positive cells and CNPase/BrdU-positive cells of MCAO + atropine group was lower(P < 0.05). Conclusions Cholinergic signal could promote NSCs differentiation in peri-infarc-tion region,a lleviate cerebral edema,and improve the brain function restoration after stroke.
