Trichinella spiralis Infection Suppressed Gut Inflammation with CD4+CD25+Foxp3+ T Cell Recruitment.
- Author:
Min Kyoung CHO
1
;
Mi Kyung PARK
;
Shin Ae KANG
;
Seon Hee CHOI
;
Soon Cheol AHN
;
Hak Sun YU
Author Information
1. Department of Parasitology, Pusan National University School of Medicine, Busan 626-813, Korea. hsyu@pusan.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Trichinella spiralis;
dextran sulfate sodium (DSS);
CD4+CD25+Foxp3+ T (Treg) cells;
intestinal inflammation
- MeSH:
Animals;
Colitis/chemically induced/*immunology/pathology;
Cytokines/genetics/*immunology/metabolism;
Dextran Sulfate/adverse effects;
Disease Models, Animal;
Female;
Forkhead Transcription Factors/immunology/metabolism;
*Gene Expression Regulation;
Larva;
Mice;
Mice, Inbred C57BL;
Spleen/immunology;
T-Lymphocytes, Regulatory/*immunology;
Trichinella spiralis/*immunology;
Trichinellosis/*immunology/parasitology
- From:The Korean Journal of Parasitology
2012;50(4):385-390
- CountryRepublic of Korea
- Language:English
-
Abstract:
In order to know the effect of pre-existing Trichinella spiralis infection on experimentally induced intestinal inflammation and immune responses, we induced colitis in T. spiralis-infected mice and observed the severity of colitis and the levels of Th1, Th2, and regulatory cytokines and recruitment of CD4+CD25+Foxp3+ T (regulatory T; Treg) cells. Female C57BL/6 mice were infected with 250 muscle larvae; after 4 weeks, induction of experimental colitis was performed using 3% dextran sulfate sodium (DSS). During the induction period, we observed severity of colitis, including weight loss and status of stool, and evaluated the disease activity index (DAI). A significantly low DAI and degree of weight loss were observed in infected mice, compared with uninfected mice. In addition, colon length in infected mice was not contracted, compared with uninfected mice. We also observed a significant increase in production of pro-inflammatory cytokines, IL-6 and IFN-gamma, in spleen lymphocytes treated with DSS; however, such an increase was not observed in infected mice treated with DSS. Of particular interest, production of regulatory cytokines, IL-10 and transforming growth factor (TGF)-beta, in spleen lymphocytes showed a significant increase in mice infected with T. spiralis. A similar result was observed in mesenteric lymph nodes (MLN). Subsets of the population of Treg cells in MLN and spleen showed significant increases in mice infected with T. spiralis. In conclusion, T. spiralis infection can inhibit the DSS-induced colitis in mice by enhancing the regulatory cytokine and Treg cells recruitment.
