Amentoflavone inhibits debris-induced osteolysis and osteoclastogenesis through blocking MAPK pathway
10.3969/j.issn.1006-5725.2018.05.005
- VernacularTitle:穗花杉双黄酮通过阻断丝裂原活化蛋白激酶通路抑制钛颗粒诱导的骨溶解及破骨细胞的生成
- Author:
Zhen ZHANG
1
;
Xiaoqi ZHOU
;
Yu SUN
;
Xiaolei LI
;
Wu ZHANG
;
Lianqi YAN
Author Information
1. 大连医科大学 辽宁大连116044
- Keywords:
aseptic loosening;
MAPK;
osteolysis;
osteoclastogenesis;
RANKL
- From:
The Journal of Practical Medicine
2018;34(5):707-711
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of amentoflavone(AMF)on inhibiting wear debris-in-duced osteolysis in vivo and RANKL-induced osteoclastogenesis in vitro. Methods Twenty-four male C57BL/J6 mice were randomly divided into four groups:the control group,titanium group,the low and high concentration group.Micro-CT and histological analysis were performed.CCK-8 assay was used to determin the effect of AMF on the proliferation of BMMs.TRAP staining and bone resorption assays were used to investigate the effect of AMF on osteoclastogenesis and function. The effect of AMF on prevention MAPK signaling was detected by Western blot assay. Results AMF could prevent osteolysis in vivo and suppress osteoclastogenesis,bone resorption without cytotoxicity in vitro.AMF suppressed MAPK signaling pathways.Conclusion AMF could inhibit osteoclastogenesis and titanium debris-induced osteolysis through suppressing MAPK signaling.
