Effect of EP4 gene silencing on the growth and migration of papillary thyroid carcinoma K1 cells
10.3969/j.issn.1006-5725.2018.05.004
- VernacularTitle:EP4基因沉默对甲状腺乳头状癌K1细胞生长及迁移的影响
- Author:
Rongyu ZHONG
1
;
Fen XU
;
Heying AI
;
Danli ZHOU
;
Xiaoying YANG
;
Liao SUN
Author Information
1. 中山大学附属第五医院内分泌与代谢病科 广东珠海519000
- Keywords:
papillary thyroid carcinoma;
prostaglandin E receptor 4;
growth;
migration
- From:
The Journal of Practical Medicine
2018;34(5):702-706
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of EP4 gene silencing on the growth and migration of K1 cells. Methods K1 cells with stable knockdown of EP4 were constructed with lentiviral vector. QRT-PCR and western blot analysis were used to detect the expression of EP4 mRNA and protein in K1 cells. CCK8 assay and flow cytometry were employed to measure cell viability and apoptosis. Transwell assay was applied to detect cell migration. Results Compared with the negative control group,the mRNA and protein expression of EP4 were sig-nificantly decreased in K1 cells with stable knockdown of EP4. Furthermore,shRNA-mediated silencing of EP4 gene remarkably suppressed cell viability and induced apoptosis of K1 cells.The migration of K1 cells with knock-down of EP4 was decreased compared with the negative control group. Conclusions EP4 gene silencing can in-hibit growth and induce apoptosis of K1 cells.Downregulation of EP4 can significantly reduce migration of K1 cells.
