Effect of knockdown or overexpression of G6PD on the proliferation and migration of hepatoma cells
10.3969/j.issn.1006-5725.2018.05.003
- VernacularTitle:敲低及过表达G6PD对肝癌细胞增殖和迁移的影响
- Author:
Xiao FENG
1
;
Zhaoyu LIU
;
La HU
;
Jitao CHEN
;
Zicheng ZENG
;
Jifang LIU
Author Information
1. 广州医科大学附属第五医院 广州510710
- Keywords:
G6PD;
hepatocellular carcinoma;
pentose phosphate pathway
- From:
The Journal of Practical Medicine
2018;34(5):698-701,706
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of knockdown or overexpression of G6PD on proliferation, growth and migration of human hepatocellular carcinoma cell PLC/PRF/5. Methods Lentivirus-mediated knock-down or overexpression of G6PD was achieved in human hepatocellular carcinoma cell line PLC/PRF/5. RT-PCR and Western blotting assay were used to detect the overexpression or knockdown of G6PD.Cell proliferation and mi-gration curves were recorded by real-time cell analysis system(RTCA),the cell proportion in the DNA replication phase can be directly displayed with EDU experiment,cell growth ability was detected by colony forming assay. Results The doubling time of cells in G6PD knockdown group was longer than that of the control group,and the cell growth rate decreased significantly,the proportion of cells in proliferative phase(43.2%)was lower than that in the control group,but the rates colony formation and migration were significantly decreased(P<0.05,respective-ly),and the migration curves separated apparently.While no significant differences in proliferation,growth and mi-gration of PLC/PRF/5 cells were found between the over-expressed strain and the control group. Conclusion The reduction of G6PD expression in HCC cells inhibits the proliferation and growth of HCC,which may lay a foun-dation for the further study of the pathogenesis and treatment of HCC.
