Varied therapeutic effects of Human Amniotic Mesenchymal Stem Cell Transplantation with Different Transplantation Methods for Spinal Cord Injury Therapy in Rats
10.3969/j.issn.1006-5725.2018.04.010
- VernacularTitle:人羊膜间充质干细胞不同移植方式治疗大鼠脊髓损伤疗效比较
- Author:
Jingjing MA
1
;
Xuyong LIAO
;
Yujie ZHAO
;
Huangfei YU
Author Information
1. 遵义医学院第三附属医院检验科 遵义563000
- Keywords:
human amniotic mesenchymal stem cells;
spinal cord injury;
transplantation;
BBB score
- From:
The Journal of Practical Medicine
2018;34(4):562-566,571
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the therapeutic effects of orthotopic injection and tail vein injection of human amniotic mesenchymal stem cells(hAMSCs)on histological restoration and neurological functions of rats with spinal cord injury. Methods Transected spinal cord injury model in rats was established by transplanting DAPI prelabelled hAMSCs one week after injury.BBB scores were used to evaluate the hindlimb movement of rats. The histological patterns.and morphology of medullary sheath of spinal cord were observed. Results BBB scores in the orthotopic injection group and tail vein injection group were increased gradually from one to six week after hAM-SCs transplantation and reached 6.5 ± 0.5 and 7.12 ± 1.61 respectively 6 weeks after cell transplantation,higher than that of the control group(both P < 0.01). However,there was no statistical significance between the two groups.Histological results indicated that the repair of injured tissue in the orthotopic injection group and tail vein injection group were both better than that in the control group,and there were more vesica and loosened layers forming in the injured spinal cord of rats in the PBS control group as compared with the orthotopic and tail vein transplantation group. Conclusion hAMSCs transplantation through tail vein injection could promote histological restoration and neurological regeneration of rats with spinal cord injury,which has the similar therapeutic effects with hAMSCs orthotopic transplantation.
