Effect of mesenchymal stem cells overexpressing il10 transplanted in rats with myocardial infarction
10.3969/j.issn.1006-5725.2018.04.009
- VernacularTitle:间充质干细胞过表达IL-10对心肌梗死模型大鼠心功能的影响
- Author:
Cheng WANG
1
;
Xia LI
;
Zhen LIU
;
Minglei HAN
;
Yonglan HOU
;
Xiaoyong QI
Author Information
1. 新乡市中心医院心血管内一科 河南新乡453000
- Keywords:
myocardial infarction;
mesenchymal stem cells;
interleukin-10
- From:
The Journal of Practical Medicine
2018;34(4):556-561
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the modulation effects of mesenchymal stem cells(MSCs)overex-pressing IL-10 transplanted in a rat model of myocardial infarction and its possible mechanism. Methods The MI rats were established by left anterior descending coronary artery ligation and the rats were then randomly divided into three groups:group C(MSC+PBS),group P(pcDNA3-IL-10+MSC),group K(pcDNA3+MSC).Echocardiography and hemodynamic examinations were used to evaluate the cardiac function.Myocardial infarction size were evaluate were evaluate by Immumohistochemical stainingmyocardial.At the same time,Immunofluorescence and western blot was applied to show the expression of Caspase-3,TNF-α and IL-1β,respectively.Results The left ventricular ejec-tion fraction and fractional shortening in three groups showed no significant difference(P>0.05)at different time;There were no statistically significant differences between the groups K and group C and the left ventricular ejection fraction,fractional shortening in group P were highest(P<0.05);The left ventricular ejection fraction(Finteractive=2.564,Pinteractive=0.015)and fractional shortening(Finteractive=2.233,Pinteractive=0.022)have interactive effect in three groups.After 4 weeks,LVSP,+dp/dtmax and-dp/dtmax in group P were significantly higher than that of C group and K group,while the LVEDP was lower(P<0.01);immunofluorescence and Western blot showed that Caspase-3, TNF-α and IL-1β in group P were significantly lower than that of C group and K group,and the difference was statis-tically significant(P<0.05).Conclusion MSC overexpressing IL-10 can promote the recovery of cardiac function after MI,which may be related to inhibition of Caspase-3 apoptosis gene and TNF-α and IL-1β inflammatory factors.
