The Application of mRNA-based gene transfer in mesenchymal stem cell-mediated migration and cytotox-icity of DBTRG glioma cells
10.3969/j.issn.1006-5725.2018.04.005
- VernacularTitle:利用体外合成的mRNA编辑间充质干细胞对神经胶质瘤DBTRG细胞杀伤作用
- Author:
Hongxia FU
1
;
Zhipeng MA
;
Chengke CHU
;
Xingrong GUO
Author Information
1. 十堰市太和医院(湖北医药学院附属医院)检验科 湖北十堰442000
- Keywords:
synthesized mRNAs;
mesenchymal stem cells;
anticancer gene;
glioma cells DBTRG;
gene therapy
- From:
The Journal of Practical Medicine
2018;34(4):535-539,547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mRNA-based anticancer gene transfer in MSCs-mediated cytotox-icity of glioma cells. Methods TRAIL mRNA and PTEN mRNA were synthesized in vitro. Immunoblotting assay was used to detect the expression of TRAIL and PTEN in the transfected MSCs.Transwell co-culture was perform to detect the migration ability of MSCs after gene transfection. The bioluminescence,live/dead staining and real time cell analyzer were used to analyze the viability of DBTRG cells. Results Compared with non-transfected MSCs, an enhanced migration rate was observed in MSCs with two kind of mRNA transfection.TRAIL-and PTEN-mRNA-induced cytotoxicity in DBTRG glioma cell was correlated with the ratio of the conditioned medium of the transfect-ed MSCs. A synergistic action was observed in TRAIL and PTEN in the transwell co-culture model. Conclusion The present study reveals the effect of synthesized mRNA-based gene transfer on mesenchymal stem cell-mediated cytotoxicity of glioma cells(DBTRG).
